DNA barcoding of Nyssaceae (Cornales) and taxonomic issues
نویسندگان
چکیده
DNA barcoding, as a tool for species discrimination, has been efficiently used in animals. However, there are still debates on which DNA region (s) can be adopted as the standard barcode (s) for land plants. In the present study, we evaluated the four proposed barcoding loci (matK, rbcL, trnH-psbA and ITS) on nine species of Nyssaceae. The results showed that ITS was the best performing single locus, although matK + rbcL might be used as the core barcodes for land plants. The chloroplast regions have low resolution compared with ITS. The low efficiency of these candidate barcodes in Nyssaceae might be caused by a poor taxonomy, especially within the genus Nyssa. The results also indicated that species status of N. shangszeensis, N. sinensis, N. shweliensis and N. wenshanensis requires to be reevaluated based on more morphological characters combined with rapidly evolving loci.
منابع مشابه
Comparative and Phylogenetic Analyses of the Complete Chloroplast Genomes of Three Arcto-Tertiary Relicts: Camptotheca acuminata, Davidia involucrata, and Nyssa sinensis
The Arcto-Tertiary relict genera, Camptotheca, Davidia, and Nyssa represent deep lineages in the asterid order Cornales. Recent phylogenetic studies suggested that these genera should be placed in a newly circumscribed family, Nyssaceae. However, because these analyses were based upon a few genes, it is prudent and necessary to examine further evidence before adopting this taxonomic treatment. ...
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